DNA sequencing of fungi in a microbiology laboratory
نویسنده
چکیده
The identification of fungi in a general microbiology laboratory using traditional techniques has always been problematic for several reasons. These include the requirement for trained personnel to identify the fungi, as well as the fact that many fungi take much longer than bacteria to grow, making identification of these organisms in a timely fashion difficult. An additional complication is that some fungi do not show characteristic structures, making identification by traditional techniques difficult, if not impossible. DNA sequencing for the identification of fungal cultures can be easily established in a laboratory that is set up for bacterial DNA sequencing. This technology, when complemented with appropriate levels of training in classical fungal identification, will extend the mycological capabilities of a general microbiology laboratory.
منابع مشابه
Study of ectomycorrhizal fungi with beech trees in highland beech forests (Farim, Mazandaran province)
In this study, the ectomycorhizal fungi from beech trees in highland beech forests of Farim (Mazandaran province) were identified based on extraction of DNA from roots and sequencing the ITS region of nuclear ribosomal DNA. For this purpose, in the altitude of 1500-2100 meters A.S.L, 30 plot and one plant per each plot were selected randomly and samples were taken from roots in depths of 10 cm ...
متن کاملIsolation and identification of Eurotium species from contaminated rice by morphology and DNA sequencing
30 milled rice samples were collected from retailers in four states of Malaysia. These samples were evaluated for Eurotium spp. contaminations by direct plating on malt extract salt agar (MESA). All Eurotium were isolated and identified based on morphology and nucleotide sequences of internal transcribed spacer 1 (ITS1) and ITS2 of the rDNA. Four Eurotium species (E. rubrum, E. amstelodami, E....
متن کاملComparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis
Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
متن کاملMolecular Testing in Microbiology
There are significant challenges associated with qualitative and quantitative nucleic acid tests performed in diagnostic laboratories. The development of internationally available certified reference materials which can be traced to reference measurements will contribute to a better understanding of the performance characteristics of nucleic acid tests and enhance reliability and comparability ...
متن کاملSilver nanoparticles, a potential alternative to conventional anti-fungal agents to fungal pathogens affecting crop plants
Metallic silver nanoparticles have been reduced from silver nitrate by employing the extracellular enzymatic machinery of edible White button Mushroom (Agaricus bisporus). The physical properties of these particles, size and shapes have been determined using techniques like TEM, FTIR and XRD and were reported in our earlier report. But, what stands of paramount importance in the presen...
متن کامل